Application

Research CategorySignaling

Detect FNIP1 using this mouse monoclonal Anti-FNIP1 Antibody, clone 1C10.2, Cat. No. MABS1717, validated for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Folliculin-interacting protein 1 (UniProt Q8TF40; FNIP1 protein) is encoded by the FNIP1 (also known as KIAA1961) gene (Gene ID 96459) in human. FNIP1 and 2 proteins are homologous partners of folliculin (FLCN) and are required for FLCN localization to lysosomes during amino acid starvation. FNIP1 protein is an evolutionary conserved 130 kDa protein that is shown to interact with AMPK and can serve as a substrate for activated AMPK. Blocking of AMPK activity is reported to reduce FNIP protein expression in HEK293 cells. FNIP1 is also involved in autophagic process where it interacts with GABARAP of the ATG8 family and knockdown of FNIP1 is shown to enhance autophagosome formation. Strong expression of FNIP1 protein is reported in the heart, liver placenta, muscle, nasal mucosa, salivary gland and uvula and moderate expression in kidney and lung. Higher levels have been reported in renal cell carcinoma (RCC). Loss of FNIP1 protein is also shown to mitigate muscle damage in a murine model of Duchenne muscular dystrophy. Ref.: Baba, M., et al. (2006). Proc. Natl. Acad. Sci. USA 103, 15552 15557. Hasumi, H., et al. (2015). Proc. Natl. Acad. Sci. USA 112, 1624-1631.

Immunogen

GST-tagged recombinant fragment corresponding to a region within the C-terminal half of human FNIP1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G purified.

Format: Purified

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FNIP1 in 10 µg of HEK293 cell lysate.

Specificity

Epitope region is present in spliced isoforms 1 and 3, but not 2, of human FNIP1 reported by UniProt (Q8TF40).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~130 kDa observed. 130.6/127.4 kDa (isoform 1/3) calculated. Uncharacterized bands may be observed in some lysate(s).